The Importance of Following the Correct Order of Draw in Phlebotomy: Preventing Sample Contamination, Misdiagnosis, and Regulatory Issues

Summary

  • Incorrect order of draw can lead to sample contamination
  • Patient misdiagnosis and treatment errors may occur
  • Regulatory consequences for healthcare facilities

Introduction

Phlebotomy is a crucial part of medical lab procedures in the United States. It involves the collection of blood samples from patients for diagnostic testing. Following the correct order of draw is essential to ensure the accuracy and quality of these samples. Failure to adhere to proper phlebotomy procedures can have serious consequences, both for the patient and the healthcare facility.

The Order of Draw

The order of draw refers to the sequence in which blood samples are collected from a patient. This sequence is important because it helps to prevent Cross-Contamination between different blood samples. The commonly accepted order of draw is as follows:

  1. Yellow or SPS tubes (sterile tubes with preservatives)
  2. Light blue tubes (containing Sodium Citrate)
  3. Red or gold tubes (no additive or Clot Activator)
  4. Green tubes (Heparin anticoagulant)
  5. Lavender or purple tubes (EDTA anticoagulant)
  6. Gray tubes (sodium fluoride preservative)

Potential Consequences of Not Following the Order of Draw

Sample Contamination

One of the most significant risks of not following the order of draw is sample contamination. When blood samples are collected out of sequence, there is a higher likelihood of carryover contamination. This can result in inaccurate Test Results and potentially mislead Healthcare Providers in their diagnosis and treatment of the patient.

Patient Misdiagnosis and Treatment Errors

Incorrect Test Results due to sample contamination can lead to patient misdiagnosis and treatment errors. For example, if a sample intended for a Blood Culture is contaminated with Anticoagulant from a lavender tube, it may falsely indicate the presence of an infection where none exists. This can lead to unnecessary treatments or medications being prescribed, which can harm the patient's health.

Regulatory Consequences for Healthcare Facilities

In addition to the impact on patient care, not following the order of draw can also have regulatory consequences for healthcare facilities. Accrediting bodies such as the College of American Pathologists (CAP) and the Clinical Laboratory Improvement Amendments (CLIA) require strict adherence to phlebotomy procedures to ensure the quality and accuracy of Test Results. Failure to comply with these guidelines can result in penalties, fines, or even loss of accreditation for the facility.

Conclusion

Following the correct order of draw in phlebotomy procedures is essential to maintain the integrity of blood samples and ensure accurate Test Results. Failure to adhere to proper phlebotomy procedures can have serious consequences for both patients and healthcare facilities in the United States. It is important for Healthcare Providers to receive adequate training and education on phlebotomy best practices to prevent these potential risks and uphold the quality of patient care.

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